《植物生理学报》 2013, 49(12): 1355-1358

钾离子对长柄扁桃不定芽诱导的影响

郭斌, 陈邦, 吴道长, 车德路, 尉亚辉*, 申烨华, 李聪

西北大学生命科学学院, 西部资源生物与现代生物技术教育部重点实验室, 陕西省生物技术重点实验室, 西安710069

通信作者：尉亚辉；E-mail: weiyahui@nwu.edu.cn；Tel: 029-88302199

摘　要：

研究了钾离子对长柄扁桃不定芽诱导的影响, 结果表明, 在MS基本培养基中添加800~1 200 mg·L^-1钾离子有利于长柄扁桃不定芽的形成和生长, 不定芽的诱导率和数量分别比对照提高了17%和84%, 不定芽的平均高度提高了64%; 高浓度钾离子(>1 600 mg·L^-1)可导致长柄扁桃不定芽严重褐化。生理指标测定结果表明, 适当浓度的钾离子提高了抗氧化酶(SOD和POD)的活性和不定芽的组织细胞活力; 高剂量的钾离子(>1 600 mg·L^-1)显著增加了不定芽中MDA的含量。

关键词：钾离子; 长柄扁桃; 不定芽

收稿：2013-06-24   修定：2013-08-27

资助：波音(中国)投资有限公司科研项目、国家自然科学基金(31000144)和陕西省教育厅科研计划项目(12JK0846)。 致谢 尹久盛老师和陈闽老师对该研究精心指导和帮助。

Effects of Potassium Ion on Adventitious Bud Induction of Amygdalus pedunculata

GUO Bin, CHEN Bang, WU Dao-Chang, CHE De-Lu, WEI Ya-Hui*, SHEN Ye-Hua, LI Cong

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi’an 710069, China

Corresponding author: WEI Ya-Hui; E-mail: weiyahui@nwu.edu.cn; Tel: 029-88302199

Abstract:

The effects of potassium ion on adventitious bud induction of Amygdalus pedunculata were studied. The results showed that the optimal concentration range of potassium ions is 800–1 200 mg·L^-1. The adventitious bud induction frequency and the bud number per explants increased by 17% and 84%, respectively, compared to those cultured in MS basic medium without additional potassium nutrition. And the height of the adventitious bud increased by 64%. However, the high concentrations of potassium ions (>1 600 mg·L^-1) caused adventitious buds serious browning. The physiological indexes suggested that appropriate potassium concentrations increased antioxidant enzymes (SOD and POD) activities and adventitious bud viability. High dose of potassium ions (>1 600 mg·L^-1) significantly increased the malondialdehyde content in adventitious bud.

Key words: potassium ion; Amygdalus pedunculata pall.; adventitious bud